Ethylenediaminetetraacetic acid (EDTA) ndi chitsulo chelating wothandizira. Zomwe zimatchedwa chelating zimatanthawuza kuti zimatha kugwirizanitsa ndi zitsulo zina zachitsulo ndikupanga zitsulo zomangirira kuti zisagwire ntchito.

Pogwiritsa ntchito khalidwe ili la EDTA, pali mitundu iwiri ya ntchito muzoyesera;

Mtundu umodzi ndi reverse application, womwe umalepheretsa zochitika zina posokoneza kumanga kwa ayoni.

Tengani ayoni: Pazinthu zina, kumangirira ma ayoni achitsulo ndikofunikira. Timaphatikiza EDTA ndi ayoni achitsulo kuti aletse izi.

Mwachitsanzo, magazi coagulation amafuna nawo calcium ayoni. Timawonjezera EDTA m'magazi kuti tiyese ma ions a calcium ndi EDTA, motero timapewa kutsekeka kwa magazi. Iyi ndi mfundo ya EDTA anticoagulation;

Mwachitsanzo, kukonzekera kwa decalcification solution mu pathological staining kumagwiritsanso ntchito chelation effect ya EDTA. Tikudziwa kuti ngati minofu ya kashiamu yapamwamba siidadulidwe, zimakhala zovuta kudula minofu yonse podula, choncho tifunika kuchepetsa tisanadule.

Palinso mtundu wogwiritsa ntchito patsogolo womwe umalimbikitsa machitidwe ena posokoneza kumangirira kwa ayoni achitsulo.

Mwachitsanzo, onjezani EDTA ku trypsin digestion solution. Ngati EDTA sinawonjezedwe, trypsin imamanga ku calcium ndi magnesium pa cell pamwamba pa cell kapena pachikhalidwe cha chikhalidwe, motero amalepheretsa ntchito ya trypsin.

Panthawiyi, onjezani pang'ono EDTA kuti EDTA igwirizane ndi calcium, magnesium ndi ma ion ena aulere. Panthawiyi, mkhalidwe wabwino umapangidwa kuti trypsin ichitike.

Chodabwitsa kwambiri pa EDTA ndikusungunuka kwake. Zimasungunuka pang'ono m'madzi ndipo zimawonekera zoyera m'madzi. Panthawiyi, onjezerani sodium hydroxide kuti musinthe pH yake. Pamene pH ikukwera, yankho limakhala lomveka bwino komanso lomveka bwino. Pamene pH ili pafupi ndi 8, imamveka bwino. Sichiwopa asidi koma alkali.

EDTA ili ndi ntchito zambiri zabwino. Tingaone kuti zinthu za m’chilengedwe n’zamatsenga kwambiri, koma sitinazizindikire.

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